Characterization of Excretory and Secretory Larval Antigen of Toxocara canis by Western Blotting
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منابع مشابه
Antigenic Characterization of Excretory / Secretory Antigen of Oesophagostomum Columbianum Using Western Blotting
In the present investigation, E/S antigen from O. columbianum was isolated by in vitro culture method and hyper immune serum was raised against this antigen in rabbits. On SDS – PAGE analysis, characterization, the E/S antigen revealed six polypeptides at 29.0, 38.0, 49.0, 71.0, 84.0 and 95.0 kDa. Further, three immuno-reactive polypeptides at 29.0, 38.0 and 49.0 kDa were detected in western bl...
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The gene encoding the excretory-secretory antigen TES-120 of dog ascarid worm Toxocara canis was cloned into the bacterium Escherichia coli. The specificity of the recombinant TES120 antigen produced by the bacterium was investigated. A total of 45 human serum samples from patients infected with different helminthes and protozoa, including 8 cases of toxocariasis, were tested against the recomb...
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reports have emphasised the severity of neurological and systemic disorder in patients with toxocaral meningoencephalitis.1 2 " Apart from neck stiffness our patient was surprisingly well with no evidence of encephalitis. Her diagnosis might have been that of an atypical viral meningitis had Romanovsky staining not been performed. We think that this investigation may be useful in culture negati...
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BACKGROUND: Toxocara canis is the most prevalent intestinal roundworm of canid species. OBJECTIVES: This study aims to evaluate the effects of Toxocara canis excretory-secretory antigens (TcES Ag) on modulating the immune system in Experimental Allergic Encephalomyelitis (EAE) model. METHODS: Adult worms of T.canis were collected from dogs to obtain excretory-secretory antigens. Female C57BL/6m...
متن کاملProteinases in Excretory-Secretory Products of Toxocara canis Second-Stage Larvae: Zymography and Modeling Insights
Components released in excretory-secretory products of Toxocara canis larvae (TES) include phosphatidylethanolamine-binding proteins (TES26), mucins (TES120, MUC2-5), and C-type lectins (TES32, TES70) and their biochemical, immunological, and diagnostic properties have been extensively studied albeit proteinase activities towards physiological substrates are almost unknown. Proteolytic activiti...
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ژورنال
عنوان ژورنال: International Journal of Current Microbiology and Applied Sciences
سال: 2019
ISSN: 2319-7692,2319-7706
DOI: 10.20546/ijcmas.2019.805.095